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KMID : 0545119940040040264
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Journal of Microbiology and Biotechnology
1994 Volume.4 No. 4 p.264 ~ p.269
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Nucleotide Sequence Analysis of the RNA-dependent RNA Poymerase Gene of Infectious Pancreatic Necrosis Virus DRT Strain
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CHUNG, HYE KYUNG
LEE, SEONG HUN/KIM, SOO YOUNG/LEE, HYUNG HOAN
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Abstract
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To determine the nucleotide sequence of the ds RNA segment B containing the RNA dependent RNA polymerase (RdRp) gene of the DRT strain of infectious pancreatic necrosis virus (IPNV), the cDNA of the ds RNA segment B of the DRT strain of IPNV was synthesized using the reverse transcriptase (RT)-polymerase chain reaction (PCR) and its cDNA nucleotide sequence was determined. The DRT segment B was 2,783 by long and contained only a single long open reading frame (ORF) of 2,535 by in length. This ORF nucleotides encoded the VP1 protein, the putative RdRp of IPNV. The VP1 protein consisted of 845 amino acids. The molecular weight of the RdRp, as deduced from the nucleotide sequence, is 94,426. The nucleotide sequence of the ORF of the DRT showed 89.7% homology to the jasper strain, but 80.8% to the Sp strain. The amino acid sequence of the ORF of the DRT showed 97.6% homology to the jasper strain, but 88.7% to the Sp strain. The conserved GTP-binding motif was detected in VP1 protein.
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